Sachse lab

Electron cryomicroscopy of autophagy complexes

Method development

Spring is a single-particle based helical reconstruction package developed in our laboratory to determine 3D structures of a variety of highly ordered and less ordered specimens with helical symmetry from electron micrographs.
spring single-particle helical reconstruction
Helical diffraction simulator is a web server tool developed in our laboratory to display Fourier patterns of helices based on input helical symmetry parameters according to the theory formulated by Cochran, Crick and Vand in 1952.
spring single-particle helical reconstruction
LocScale is density scaling (sharpening) procedure that aims to enhance interpretability of cryo-EM density maps. LocScale is a reference-based local amplitude scaling tool using prior model information to improve contrast of cryo-EM density maps.
spring single-particle helical reconstruction