Sachse lab

Electron cryomicroscopy of autophagy complexes

Method development

Spring is a single-particle based helical reconstruction package developed in our laboratory to determine 3D structures of a variety of highly ordered and less ordered specimens with helical symmetry from electron micrographs.
spring single-particle helical reconstruction
LocScale is density scaling (sharpening) procedure that aims to enhance interpretability of cryo-EM density maps. LocScale is a reference-based local amplitude scaling tool using prior model information to improve contrast of cryo-EM density maps.
spring single-particle helical reconstruction