Sachse lab

Electron cryomicroscopy of autophagy complexes

Lab members

Carsten Sachse

Carsten Sachse
Carsten Sachse. I am currently a group leader at the EMBL. I got in touch with electron cryomicroscopy by studying amyloid fibrils in the groups of Marcus Fändrich (now in Ulm, Germany) and Niko Grigorieff (now at Janelia Farm, USA). For my postdoctoral period, I went to the MRC Laboratory of Molecular Biology (Cambridge, UK) to work with Richard Henderson and Roger Williams. In the last few years, we set up a group to study the mechanisms of autophagy by electron cryomicroscopy. Traditionally, I have been interested in the advancement of high-resolution single-particle EM with a particular focus on helical reconstruction. These developments have lead to our recent helical processing software package Spring.

Mairi Clarke

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Mairi Clarke. I am currently research technician in the Sachse group. I started working at EMBL in 1995 for Stephen Fuller in SCB where I learned the fundamentals of sample preparation for cryo-TEM.
In 2014 I returned to EMBL, having spent some time at Glasgow University working as a post-doc in the fields of vesicular trafficking and cancer immunology.
I have returned to SCB and, in addition to looking after the lab organization, I am involved in expression and purification of autophagy proteins for TEM and cryo-TEM.

Laura Woodward

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Laura Woodward. I joined the lab as a postdoc at the beginning of November 2017. I obtained a BSc (Hons) in Biochemistry with German at the University of Manchester, during which I worked as an Erasmus intern in the lab of Marius Lemberg at the ZMBH, University of Heidelberg and completed my Bachelor project in the lab of Andrew Munro. I recently completed my PhD in the lab of Jim Naismith at the University of St Andrews. The focus of my PhD was determining the structures of lipopolysaccharide biosynthetic enzymes using x-ray crystallography and cryo-EM with a particular focus on membrane proteins.

Simon Fromm

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Simon Fromm. I am a Postdoc after having completed a PhD recently. I joined the lab in July 2013. My main research focus is to gain structural knowledge about TDP-43 aggregates which are implicated in several neurodegenerative diseases like amyotrophic lateral sclerosis (ALS) or frontotemporal dementia (FTD). Besides that I'm also interested in methods development and try to improve various aspects of structure determination by cryo-EM. Before coming to EMBL, I got my diploma in biochemistry (comparable to a master degree) at the University of Tübingen, Germany. During my studies and for my diploma thesis I worked in the lab of Remco Sprangers (MPI Tübingen, Germany) and got a strong interest in structural biology. I applied different biophysical methods like NMR spectroscopy and ITC to study protein complexes involved in mRNA decay.

Stefan Huber

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Stefan Huber. I am pursuing my master's degree in Molecular Biotechnology at Ruprecht-Karls University in Heidelberg. Previous work was on gene regulatory network analysis at DKFZ (Carl Herrmann) in Heidelberg, and on functional and structural connectivity analysis in neuron cultures using microelectrode arrays (group of Urs Frey at RIKEN, Kobe). At Sachse lab, I work on methods development for automated tracing of helical specimen in cryo-electron micrographs.

Maximilian Beckers

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Maximilian Beckers. I am a PhD-student and joined the group in August 2016. With degrees both in mathematics and biochemistry my main interests are in both frequentist and bayesian statistical method development for biophysical data, especially for structure determination of macromeluclar complexes. My previous work was carried out in the group of Jens Michaelis at Ulm University where I worked in the FRETnps development team with a focus on MCMC methods and also at Boehringer Ingelheim in the Computational Chemistry group with a focus on molecular dynamics simulations and trajectory analysis.

Felix Weis

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Felix Weis. I joined EMBL in April 2017. I am an electron cryo-microscopy support scientist and together with my colleague Wim Hagen, we take care of the SCB electron microscopes, providing help, training and support to internal users as well as visitors of the facility. I completed my PhD on structural study of bacterial ribosome complexes by cryo-EM in Rennes (France) in 2010 and then moved to Cambridge (UK) for a six years post-doc at the MRC Laboratory of Molecular Biology, studying from a structural point of view eukaryotic ribosome biogenesis and translation initiation regulation, still with electron microscopes. My research interests cover many aspects of cryo-EM, including sample preparation, imaging and processing.

Alumni

Chiara Bertipaglia (PhD student), now Postdoc at Columbia University (US)
Jaclyn Chan (Lab technician), now in Perth (AUS)
Rodolfo Ciuffa (PhD student), now Postdoc at ETH Zürich (CH)
Ambroise Desfosses (Postdoc), now Postdoc at IBS Grenoble (FR)
Audrey Guesdon (Postdoc), now in Montpellier (FR)
Marie Kirsten (Postdoc), now at Nspm Ltd (CH)
Goran Kokic (Internship 2014), now at Max Planck Institute of Biophysical Chemistry Göttingen (DE)
Arjen Jakobi (Postdoc), now Assistent Professor at Bionanoscience TU Delft (NL)
Jan Kosinski (Postdoc), now Group Leader at EMBL Hamburg/CSSB (DE)
Tanja Kuhm (Internship 2016 and Master thesis 2017), now at Heidelberg University (DE)
Lena Muckenfuss (Internship 2016), now at Zürich University (CH)
Arvind Ravichandran (Postdoc), now in India
Sofia Rybina (Lab technician), now in Heidelberg (DE)
Sarah Schneider (PhD student), now Postdoc at Imperial College London (UK)
Abul Tarafder (Lab manager 2012 - 2017), now Investigator Scientist at Oxford University (Sir William Dunn School of Pathology) (UK)
Shirley Tremel (Master student 2016), now PhD at MRC-LMB Cambridge (UK)
Willem Tichelaar (Postdoc), now in Heidelberg (DE)